Projection of cells across datasets

Scarf allows projections (aka mapping) of cells from one dataset to another. Such projection can help in understanding how cells are related between the two datasets. Projection/mapping is a lightweight alternative to full-blown data integration which focuses on biological interpretation. In this notebook we use data from Kang et. al.. We have already preprocessed the raw count matrix to generate UMAPs and clustering of the data (notebook here). We will use two datasets from this study: control and IFN-B treated PBMCs.

[1]:
%config InlineBackend.figure_format = 'retina'
%load_ext autotime

import scarf
scarf.__version__
[1]:
'0.7.8'
time: 1.54 s (started: 2021-08-22 17:57:56 +00:00)

1) Fetch datasets in Zarr format

[2]:
scarf.fetch_dataset('kang_15K_pbmc_rnaseq', save_path='scarf_datasets', as_zarr=True)
scarf.fetch_dataset('kang_14K_ifnb-pbmc_rnaseq', save_path='scarf_datasets', as_zarr=True)
INFO: Download started...
--2021-08-22 17:58:03--  https://files.de-1.osf.io/v1/resources/zeupv/providers/osfstorage/6097185f5dfcb90054bf8d3a
Resolving files.de-1.osf.io (files.de-1.osf.io)... 35.186.249.111
Connecting to files.de-1.osf.io (files.de-1.osf.io)|35.186.249.111|:443... connected.
HTTP request sent, awaiting response... 302 Found
Location: https://storage.googleapis.com/cos-osf-prod-files-de-1/92d89fdae4a3edd779343681655a0c41303eace73f9eefed57667fabc99b7620?response-content-disposition=attachment%3B%20filename%3D%22data.zarr.tar.gz%22%3B%20filename%2A%3DUTF-8%27%27data.zarr.tar.gz&GoogleAccessId=files-de-1%40cos-osf-prod.iam.gserviceaccount.com&Expires=1629655144&Signature=Whr55UZEiwt2C1mxK2phvdV1Kw04jUi1v7ngW9KWKTaSGKsujEXiP1HgyeuUXonG5q7Wx1e%2FRAFnlxUQRc6VXoXM8bhR23qf5LhTDJSmq%2Fxf5gnjXlcEJ1g%2F4kKOfNLYeuVh5HAcffD18AAGJDcXl8er5RbD98TCyoBGek6aqga29Ipez401T97%2B6wGc1tLqm9S%2BAkQymdEZrb2hKSmrsNg0ZQi0DRhPqgO2AiwkrQrJw0lC4O13BWCilxkFpo0WfivscieXEIyFoGgDzJK5UXhrFx66XTQlIMJv0x9V7Q6%2Fkai2OvQIQZaDJ3uhuiFtY8cHFRYRYhbDXKm4jwI00A%3D%3D [following]
--2021-08-22 17:58:04--  https://storage.googleapis.com/cos-osf-prod-files-de-1/92d89fdae4a3edd779343681655a0c41303eace73f9eefed57667fabc99b7620?response-content-disposition=attachment%3B%20filename%3D%22data.zarr.tar.gz%22%3B%20filename%2A%3DUTF-8%27%27data.zarr.tar.gz&GoogleAccessId=files-de-1%40cos-osf-prod.iam.gserviceaccount.com&Expires=1629655144&Signature=Whr55UZEiwt2C1mxK2phvdV1Kw04jUi1v7ngW9KWKTaSGKsujEXiP1HgyeuUXonG5q7Wx1e%2FRAFnlxUQRc6VXoXM8bhR23qf5LhTDJSmq%2Fxf5gnjXlcEJ1g%2F4kKOfNLYeuVh5HAcffD18AAGJDcXl8er5RbD98TCyoBGek6aqga29Ipez401T97%2B6wGc1tLqm9S%2BAkQymdEZrb2hKSmrsNg0ZQi0DRhPqgO2AiwkrQrJw0lC4O13BWCilxkFpo0WfivscieXEIyFoGgDzJK5UXhrFx66XTQlIMJv0x9V7Q6%2Fkai2OvQIQZaDJ3uhuiFtY8cHFRYRYhbDXKm4jwI00A%3D%3D
Resolving storage.googleapis.com (storage.googleapis.com)... 172.217.4.48, 172.217.4.80, 172.217.4.208, ...
Connecting to storage.googleapis.com (storage.googleapis.com)|172.217.4.48|:443... connected.
HTTP request sent, awaiting response... 200 OK
Length: 54198050 (52M) [application/octet-stream]
Saving to: ‘scarf_datasets/kang_15K_pbmc_rnaseq/data.zarr.tar.gz’

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 11850K .......... .......... .......... .......... .......... 22%  535K 3s
 11900K .......... .......... .......... .......... .......... 22% 61.8M 3s
 11950K .......... .......... .......... .......... .......... 22%  179M 3s
 12000K .......... .......... .......... .......... .......... 22% 86.2M 3s
 12050K .......... .......... .......... .......... .......... 22%  183M 3s
 12100K .......... .......... .......... .......... .......... 22%  215M 3s
 12150K .......... .......... .......... .......... .......... 23%  159M 3s
 12200K .......... .......... .......... .......... .......... 23% 81.5M 3s
 12250K .......... .......... .......... .......... .......... 23% 33.4M 3s
 12300K .......... .......... .......... .......... .......... 23%  174M 3s
 12350K .......... .......... .......... .......... .......... 23%  187M 3s
 12400K .......... .......... .......... .......... .......... 23%  210M 3s
 12450K .......... .......... .......... .......... .......... 23%  184M 3s
 12500K .......... .......... .......... .......... .......... 23%  218M 3s
 12550K .......... .......... .......... .......... .......... 23%  164M 3s
 12600K .......... .......... .......... .......... .......... 23%  191M 3s
 12650K .......... .......... .......... .......... .......... 23%  206M 3s
 12700K .......... .......... .......... .......... .......... 24%  210M 3s
 12750K .......... .......... .......... .......... .......... 24%  187M 3s
 12800K .......... .......... .......... .......... .......... 24%  218M 3s
 12850K .......... .......... .......... .......... .......... 24%  219M 3s
 12900K .......... .......... .......... .......... .......... 24%  214M 3s
 12950K .......... .......... .......... .......... .......... 24%  179M 3s
 13000K .......... .......... .......... .......... .......... 24%  219M 3s
 13050K .......... .......... .......... .......... .......... 24% 8.24M 3s
 13100K .......... .......... .......... .......... .......... 24% 12.7M 3s
 13150K .......... .......... .......... .......... .......... 24%  195M 3s
 13200K .......... .......... .......... .......... .......... 25%  186M 3s
 13250K .......... .......... .......... .......... .......... 25%  186M 3s
 13300K .......... .......... .......... .......... .......... 25%  220M 3s
 13350K .......... .......... .......... .......... .......... 25%  172M 3s
 13400K .......... .......... .......... .......... .......... 25%  189M 3s
 13450K .......... .......... .......... .......... .......... 25%  215M 3s
 13500K .......... .......... .......... .......... .......... 25%  214M 3s
 13550K .......... .......... .......... .......... .......... 25%  192M 3s
 13600K .......... .......... .......... .......... .......... 25%  192M 3s
 13650K .......... .......... .......... .......... .......... 25%  213M 3s
 13700K .......... .......... .......... .......... .......... 25% 23.4M 3s
 13750K .......... .......... .......... .......... .......... 26%  163M 3s
 13800K .......... .......... .......... .......... .......... 26%  211M 3s
 13850K .......... .......... .......... .......... .......... 26%  207M 3s
 13900K .......... .......... .......... .......... .......... 26%  353K 3s
 13950K .......... .......... .......... .......... .......... 26% 19.6M 3s
 14000K .......... .......... .......... .......... .......... 26%  162M 3s
 14050K .......... .......... .......... .......... .......... 26% 21.5M 3s
 14100K .......... .......... .......... .......... .......... 26%  182M 3s
 14150K .......... .......... .......... .......... .......... 26%  191M 3s
 14200K .......... .......... .......... .......... .......... 26%  168M 3s
 14250K .......... .......... .......... .......... .......... 27%  189M 3s
 14300K .......... .......... .......... .......... .......... 27%  183M 3s
 14350K .......... .......... .......... .......... .......... 27%  213M 3s
 14400K .......... .......... .......... .......... .......... 27%  179M 3s
 14450K .......... .......... .......... .......... .......... 27%  164M 3s
 14500K .......... .......... .......... .......... .......... 27%  212M 3s
 14550K .......... .......... .......... .......... .......... 27%  191M 3s
 14600K .......... .......... .......... .......... .......... 27%  182M 3s
 14650K .......... .......... .......... .......... .......... 27%  188M 3s
 14700K .......... .......... .......... .......... .......... 27%  203M 3s
 14750K .......... .......... .......... .......... .......... 27%  211M 3s
 14800K .......... .......... .......... .......... .......... 28%  173M 3s
 14850K .......... .......... .......... .......... .......... 28%  199M 3s
 14900K .......... .......... .......... .......... .......... 28%  200M 3s
 14950K .......... .......... .......... .......... .......... 28%  206M 3s
 15000K .......... .......... .......... .......... .......... 28%  180M 3s
 15050K .......... .......... .......... .......... .......... 28% 4.42M 3s
 15100K .......... .......... .......... .......... .......... 28% 6.81M 3s
 15150K .......... .......... .......... .......... .......... 28% 8.77M 3s
 15200K .......... .......... .......... .......... .......... 28% 17.4M 3s
 15250K .......... .......... .......... .......... .......... 28%  189M 3s
 15300K .......... .......... .......... .......... .......... 29%  203M 3s
 15350K .......... .......... .......... .......... .......... 29%  205M 3s
 15400K .......... .......... .......... .......... .......... 29%  169M 3s
 15450K .......... .......... .......... .......... .......... 29%  203M 3s
 15500K .......... .......... .......... .......... .......... 29%  214M 3s
 15550K .......... .......... .......... .......... .......... 29%  204M 3s
 15600K .......... .......... .......... .......... .......... 29%  195M 3s
 15650K .......... .......... .......... .......... .......... 29%  218M 3s
 15700K .......... .......... .......... .......... .......... 29% 6.53M 3s
 15750K .......... .......... .......... .......... .......... 29% 18.9M 3s
 15800K .......... .......... .......... .......... .......... 29% 15.6M 3s
 15850K .......... .......... .......... .......... .......... 30% 25.8M 3s
 15900K .......... .......... .......... .......... .......... 30%  205M 3s
 15950K .......... .......... .......... .......... .......... 30%  490K 3s
 16000K .......... .......... .......... .......... .......... 30% 59.7M 3s
 16050K .......... .......... .......... .......... .......... 30%  203M 3s
 16100K .......... .......... .......... .......... .......... 30%  187M 3s
 16150K .......... .......... .......... .......... .......... 30%  197M 3s
 16200K .......... .......... .......... .......... .......... 30%  166M 3s
 16250K .......... .......... .......... .......... .......... 30% 71.9M 3s
 16300K .......... .......... .......... .......... .......... 30%  195M 3s
 16350K .......... .......... .......... .......... .......... 30% 82.1M 3s
 16400K .......... .......... .......... .......... .......... 31%  189M 3s
 16450K .......... .......... .......... .......... .......... 31%  195M 3s
 16500K .......... .......... .......... .......... .......... 31%  180M 3s
 16550K .......... .......... .......... .......... .......... 31%  219M 3s
 16600K .......... .......... .......... .......... .......... 31%  166M 3s
 16650K .......... .......... .......... .......... .......... 31%  198M 3s
 16700K .......... .......... .......... .......... .......... 31%  208M 3s
 16750K .......... .......... .......... .......... .......... 31%  188M 3s
 16800K .......... .......... .......... .......... .......... 31%  177M 3s
 16850K .......... .......... .......... .......... .......... 31%  200M 3s
 16900K .......... .......... .......... .......... .......... 32%  194M 3s
 16950K .......... .......... .......... .......... .......... 32%  209M 3s
 17000K .......... .......... .......... .......... .......... 32%  168M 3s
 17050K .......... .......... .......... .......... .......... 32% 11.0M 3s
 17100K .......... .......... .......... .......... .......... 32% 18.6M 3s
 17150K .......... .......... .......... .......... .......... 32% 20.4M 3s
 17200K .......... .......... .......... .......... .......... 32% 4.84M 3s
 17250K .......... .......... .......... .......... .......... 32% 21.0M 3s
 17300K .......... .......... .......... .......... .......... 32%  204M 3s
 17350K .......... .......... .......... .......... .......... 32%  208M 3s
 17400K .......... .......... .......... .......... .......... 32%  173M 3s
 17450K .......... .......... .......... .......... .......... 33%  205M 3s
 17500K .......... .......... .......... .......... .......... 33%  216M 3s
 17550K .......... .......... .......... .......... .......... 33%  201M 3s
 17600K .......... .......... .......... .......... .......... 33%  186M 3s
 17650K .......... .......... .......... .......... .......... 33%  216M 3s
 17700K .......... .......... .......... .......... .......... 33%  210M 3s
 17750K .......... .......... .......... .......... .......... 33% 15.7M 3s
 17800K .......... .......... .......... .......... .......... 33% 17.2M 3s
 17850K .......... .......... .......... .......... .......... 33% 19.7M 3s
 17900K .......... .......... .......... .......... .......... 33% 36.8M 3s
 17950K .......... .......... .......... .......... .......... 34%  208M 3s
 18000K .......... .......... .......... .......... .......... 34%  393K 3s
 18050K .......... .......... .......... .......... .......... 34% 63.0M 3s
 18100K .......... .......... .......... .......... .......... 34% 87.8M 3s
 18150K .......... .......... .......... .......... .......... 34%  194M 3s
 18200K .......... .......... .......... .......... .......... 34% 79.3M 3s
 18250K .......... .......... .......... .......... .......... 34% 56.4M 3s
 18300K .......... .......... .......... .......... .......... 34% 70.1M 3s
 18350K .......... .......... .......... .......... .......... 34%  152M 3s
 18400K .......... .......... .......... .......... .......... 34%  189M 3s
 18450K .......... .......... .......... .......... .......... 34% 29.8M 3s
 18500K .......... .......... .......... .......... .......... 35%  191M 3s
 18550K .......... .......... .......... .......... .......... 35%  198M 3s
 18600K .......... .......... .......... .......... .......... 35%  163M 3s
 18650K .......... .......... .......... .......... .......... 35%  170M 3s
 18700K .......... .......... .......... .......... .......... 35%  216M 3s
 18750K .......... .......... .......... .......... .......... 35%  206M 3s
 18800K .......... .......... .......... .......... .......... 35%  182M 3s
 18850K .......... .......... .......... .......... .......... 35%  185M 3s
 18900K .......... .......... .......... .......... .......... 35%  211M 3s
 18950K .......... .......... .......... .......... .......... 35%  208M 3s
 19000K .......... .......... .......... .......... .......... 35%  161M 3s
 19050K .......... .......... .......... .......... .......... 36%  225M 3s
 19100K .......... .......... .......... .......... .......... 36%  204M 3s
 19150K .......... .......... .......... .......... .......... 36%  202M 3s
 19200K .......... .......... .......... .......... .......... 36%  201M 3s
 19250K .......... .......... .......... .......... .......... 36%  187M 3s
 19300K .......... .......... .......... .......... .......... 36%  202M 3s
 19350K .......... .......... .......... .......... .......... 36%  214M 3s
 19400K .......... .......... .......... .......... .......... 36%  183M 3s
 19450K .......... .......... .......... .......... .......... 36% 6.79M 3s
 19500K .......... .......... .......... .......... .......... 36% 19.6M 3s
 19550K .......... .......... .......... .......... .......... 37% 5.99M 3s
 19600K .......... .......... .......... .......... .......... 37% 16.3M 3s
 19650K .......... .......... .......... .......... .......... 37% 20.7M 3s
 19700K .......... .......... .......... .......... .......... 37% 50.5M 3s
 19750K .......... .......... .......... .......... .......... 37%  204M 3s
 19800K .......... .......... .......... .......... .......... 37%  176M 3s
 19850K .......... .......... .......... .......... .......... 37%  205M 3s
 19900K .......... .......... .......... .......... .......... 37%  212M 3s
 19950K .......... .......... .......... .......... .......... 37%  208M 3s
 20000K .......... .......... .......... .......... .......... 37%  192M 3s
 20050K .......... .......... .......... .......... .......... 37%  198M 2s
 20100K .......... .......... .......... .......... .......... 38%  214M 2s
 20150K .......... .......... .......... .......... .......... 38%  199M 2s
 20200K .......... .......... .......... .......... .......... 38%  176M 2s
 20250K .......... .......... .......... .......... .......... 38%  202M 2s
 20300K .......... .......... .......... .......... .......... 38%  205M 2s
 20350K .......... .......... .......... .......... .......... 38%  215M 2s
 20400K .......... .......... .......... .......... .......... 38%  186M 2s
 20450K .......... .......... .......... .......... .......... 38%  218M 2s
 20500K .......... .......... .......... .......... .......... 38% 5.91M 2s
 20550K .......... .......... .......... .......... .......... 38%  185M 2s
 20600K .......... .......... .......... .......... .......... 39%  170M 2s
 20650K .......... .......... .......... .......... .......... 39%  221M 2s
 20700K .......... .......... .......... .......... .......... 39%  207M 2s
 20750K .......... .......... .......... .......... .......... 39%  211M 2s
 20800K .......... .......... .......... .......... .......... 39%  199M 2s
 20850K .......... .......... .......... .......... .......... 39%  220M 2s
 20900K .......... .......... .......... .......... .......... 39%  205M 2s
 20950K .......... .......... .......... .......... .......... 39% 57.7M 2s
 21000K .......... .......... .......... .......... .......... 39%  164M 2s
 21050K .......... .......... .......... .......... .......... 39%  225M 2s
 21100K .......... .......... .......... .......... .......... 39%  221M 2s
 21150K .......... .......... .......... .......... .......... 40%  202M 2s
 21200K .......... .......... .......... .......... .......... 40%  185M 2s
 21250K .......... .......... .......... .......... .......... 40%  215M 2s
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 21350K .......... .......... .......... .......... .......... 40%  212M 2s
 21400K .......... .......... .......... .......... .......... 40%  178M 2s
 21450K .......... .......... .......... .......... .......... 40%  196M 2s
 21500K .......... .......... .......... .......... .......... 40%  207M 2s
 21550K .......... .......... .......... .......... .......... 40%  213M 2s
 21600K .......... .......... .......... .......... .......... 40%  196M 2s
 21650K .......... .......... .......... .......... .......... 40%  202M 2s
 21700K .......... .......... .......... .......... .......... 41%  220M 2s
 21750K .......... .......... .......... .......... .......... 41%  440K 2s
 21800K .......... .......... .......... .......... .......... 41% 58.6M 2s
 21850K .......... .......... .......... .......... .......... 41%  158M 2s
 21900K .......... .......... .......... .......... .......... 41%  190M 2s
 21950K .......... .......... .......... .......... .......... 41%  186M 2s
 22000K .......... .......... .......... .......... .......... 41%  179M 2s
 22050K .......... .......... .......... .......... .......... 41%  189M 2s
 22100K .......... .......... .......... .......... .......... 41%  218M 2s
 22150K .......... .......... .......... .......... .......... 41%  206M 2s
 22200K .......... .......... .......... .......... .......... 42%  220M 2s
 22250K .......... .......... .......... .......... .......... 42%  175M 2s
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 22350K .......... .......... .......... .......... .......... 42%  223M 2s
 22400K .......... .......... .......... .......... .......... 42%  210M 2s
 22450K .......... .......... .......... .......... .......... 42% 7.42M 2s
 22500K .......... .......... .......... .......... .......... 42% 8.35M 2s
 22550K .......... .......... .......... .......... .......... 42% 11.5M 2s
 22600K .......... .......... .......... .......... .......... 42%  196M 2s
 22650K .......... .......... .......... .......... .......... 42%  177M 2s
 22700K .......... .......... .......... .......... .......... 42%  217M 2s
 22750K .......... .......... .......... .......... .......... 43%  196M 2s
 22800K .......... .......... .......... .......... .......... 43%  219M 2s
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 23000K .......... .......... .......... .......... .......... 43%  200M 2s
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 23150K .......... .......... .......... .......... .......... 43%  202M 2s
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 23250K .......... .......... .......... .......... .......... 44%  182M 2s
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 23450K .......... .......... .......... .......... .......... 44%  178M 2s
 23500K .......... .......... .......... .......... .......... 44%  211M 2s
 23550K .......... .......... .......... .......... .......... 44%  208M 2s
 23600K .......... .......... .......... .......... .......... 44% 4.67M 2s
 23650K .......... .......... .......... .......... .......... 44% 6.95M 2s
 23700K .......... .......... .......... .......... .......... 44% 5.25M 2s
 23750K .......... .......... .......... .......... .......... 44% 21.2M 2s
 23800K .......... .......... .......... .......... .......... 45% 28.7M 2s
 23850K .......... .......... .......... .......... .......... 45%  168M 2s
 23900K .......... .......... .......... .......... .......... 45%  201M 2s
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 24250K .......... .......... .......... .......... .......... 45%  170M 2s
 24300K .......... .......... .......... .......... .......... 46%  199M 2s
 24350K .......... .......... .......... .......... .......... 46%  216M 2s
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 24500K .......... .......... .......... .......... .......... 46%  215M 2s
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 24600K .......... .......... .......... .......... .......... 46% 5.90M 2s
 24650K .......... .......... .......... .......... .......... 46% 16.7M 2s
 24700K .......... .......... .......... .......... .......... 46% 20.4M 2s
 24750K .......... .......... .......... .......... .......... 46% 18.0M 2s
 24800K .......... .......... .......... .......... .......... 46% 17.6M 2s
 24850K .......... .......... .......... .......... .......... 47% 29.0M 2s
 24900K .......... .......... .......... .......... .......... 47%  196M 2s
 24950K .......... .......... .......... .......... .......... 47%  216M 2s
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 25050K .......... .......... .......... .......... .......... 47%  174M 2s
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 25200K .......... .......... .......... .......... .......... 47%  223M 2s
 25250K .......... .......... .......... .......... .......... 47%  195M 2s
 25300K .......... .......... .......... .......... .......... 47%  222M 2s
 25350K .......... .......... .......... .......... .......... 47%  222M 2s
 25400K .......... .......... .......... .......... .......... 48%  210M 2s
 25450K .......... .......... .......... .......... .......... 48%  180M 2s
 25500K .......... .......... .......... .......... .......... 48%  214M 2s
 25550K .......... .......... .......... .......... .......... 48%  713K 2s
 25600K .......... .......... .......... .......... .......... 48% 60.9M 2s
 25650K .......... .......... .......... .......... .......... 48%  206M 2s
 25700K .......... .......... .......... .......... .......... 48% 87.3M 2s
 25750K .......... .......... .......... .......... .......... 48% 95.3M 2s
 25800K .......... .......... .......... .......... .......... 48% 56.6M 2s
 25850K .......... .......... .......... .......... .......... 48% 94.4M 2s
 25900K .......... .......... .......... .......... .......... 49% 95.0M 2s
 25950K .......... .......... .......... .......... .......... 49%  182M 2s
 26000K .......... .......... .......... .......... .......... 49% 76.1M 2s
 26050K .......... .......... .......... .......... .......... 49% 77.1M 2s
 26100K .......... .......... .......... .......... .......... 49% 22.7M 2s
 26150K .......... .......... .......... .......... .......... 49% 50.5M 2s
 26200K .......... .......... .......... .......... .......... 49%  178M 2s
 26250K .......... .......... .......... .......... .......... 49%  194M 2s
 26300K .......... .......... .......... .......... .......... 49%  201M 2s
 26350K .......... .......... .......... .......... .......... 49%  207M 2s
 26400K .......... .......... .......... .......... .......... 49%  186M 2s
 26450K .......... .......... .......... .......... .......... 50% 34.2M 2s
 26500K .......... .......... .......... .......... .......... 50% 17.9M 2s
 26550K .......... .......... .......... .......... .......... 50% 20.2M 2s
 26600K .......... .......... .......... .......... .......... 50%  104M 2s
 26650K .......... .......... .......... .......... .......... 50%  209M 2s
 26700K .......... .......... .......... .......... .......... 50% 9.93M 2s
 26750K .......... .......... .......... .......... .......... 50%  190M 2s
 26800K .......... .......... .......... .......... .......... 50%  175M 2s
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 27300K .......... .......... .......... .......... .......... 51% 64.4M 2s
 27350K .......... .......... .......... .......... .......... 51%  196M 2s
 27400K .......... .......... .......... .......... .......... 51% 78.7M 2s
 27450K .......... .......... .......... .......... .......... 51% 72.5M 2s
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 43000K ........
INFO: Download finished! File saved here: scarf_datasets/kang_15K_pbmc_rnaseq/data.zarr.tar.gz
.. .......... .......... .......... .......... 81%  781K 1s
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 51800K .......... .......... .......... .......... .......... 97%  189M 0s
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 51900K .......... .......... .......... .......... .......... 98%  222M 0s
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 52300K .......... .......... .......... .......... .......... 98%  217M 0s
 52350K .......... .......... .......... .......... .......... 99% 5.56M 0s
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 52450K .......... .......... .......... .......... .......... 99% 21.6M 0s
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 52550K .......... .......... .......... .......... .......... 99%  188M 0s
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 52850K .......... .......... .......... .......... .......... 99%  206M 0s
 52900K .......... .......... .......                         100%  188M=2.6s

2021-08-22 17:58:07 (19.7 MB/s) - ‘scarf_datasets/kang_15K_pbmc_rnaseq/data.zarr.tar.gz’ saved [54198050/54198050]

INFO: Download started...
--2021-08-22 17:58:09--  https://files.de-1.osf.io/v1/resources/zeupv/providers/osfstorage/6097183c5533b405cfe228b5
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Length: 60301820 (58M) [application/octet-stream]
Saving to: ‘scarf_datasets/kang_14K_ifnb-pbmc_rnaseq/data.zarr.tar.gz’

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  1100K .......... .......... .......... .......... ..........  1%  152M 6s
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  1200K .......... .......... .......... .......... ..........  2%  146M 5s
  1250K .......... .......... .......... .......... ..........  2% 31.8M 5s
  1300K .......... .......... .......... .......... ..........  2% 60.4M 5s
  1350K .......... .......... .......... .......... ..........  2% 12.6M 5s
  1400K .......... .......... .......... .......... ..........  2%  151M 5s
  1450K .......... .......... .......... .......... ..........  2%  146M 5s
  1500K .......... .......... .......... .......... ..........  2% 14.0M 5s
  1550K .......... .......... .......... .......... ..........  2% 24.9M 5s
  1600K .......... .......... .......... .......... ..........  2% 37.2M 4s
  1650K .......... .......... .......... .......... ..........  2% 36.8M 4s
  1700K .......... .......... .......... .......... ..........  2% 17.3M 4s
  1750K .......... .......... .......... .......... ..........  3% 76.4M 4s
  1800K .......... .......... .......... .......... ..........  3% 96.7M 4s
  1850K .......... .......... .......... .......... ..........  3%  155M 4s
  1900K .......... .......... .......... .......... ..........  3%  162M 4s
  1950K .......... .......... .......... .......... ..........  3%  125M 4s
  2000K .......... .......... .......... .......... ..........  3%  133M 4s
  2050K .......... .......... .......... .......... ..........  3%  153M 4s
  2100K .......... .......... .......... .......... ..........  3%  178M 4s
  2150K .......... .......... .......... .......... ..........  3%  140M 4s
  2200K .......... .......... .......... .......... ..........  3% 85.3M 3s
  2250K .......... .......... .......... .......... ..........  3%  155M 3s
  2300K .......... .......... .......... .......... ..........  3% 12.5M 3s
  2350K .......... .......... .......... .......... ..........  4%  142M 3s
  2400K .......... .......... .......... .......... ..........  4% 82.0M 3s
  2450K .......... .......... .......... .......... ..........  4%  154M 3s
  2500K .......... .......... .......... .......... ..........  4%  158M 3s
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  2800K .......... .......... .......... .......... ..........  4%  152M 3s
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  2900K .......... .......... .......... .......... ..........  5%  180M 3s
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  3000K .......... .......... .......... .......... ..........  5%  201M 3s
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  3250K .......... .......... .......... .......... ..........  5%  225M 2s
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  3500K .......... .......... .......... .......... ..........  6%  200M 2s
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  3750K .......... .......... .......... .......... ..........  6% 13.1M 2s
  3800K .......... .......... .......... .......... ..........  6% 95.0M 2s
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  4250K .......... .......... .......... .......... ..........  7% 50.1M 2s
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  4900K .......... .......... .......... .......... ..........  8%  155M 2s
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  5000K .......... .......... .......... .......... ..........  8% 11.5M 2s
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  5100K .......... .......... .......... .......... ..........  8% 37.9M 2s
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  5250K .......... .......... .......... .......... ..........  9% 94.0M 2s
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  5500K .......... .......... .......... .......... ..........  9% 21.0M 2s
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  5700K .......... .......... .......... .......... ..........  9% 94.6M 2s
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  5950K .......... .......... .......... .......... .......... 10% 21.9M 2s
  6000K .......... .......... .......... .......... .......... 10% 13.7M 2s
  6050K .......... .......... .......... .......... .......... 10% 16.9M 2s
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  6700K .......... .......... .......... .......... .......... 11% 13.9M 2s
  6750K .......... .......... .......... .......... .......... 11% 32.5M 2s
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  7050K .......... .......... .......... .......... .......... 12% 98.5M 2s
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  7750K .......... .......... .......... .......... .......... 13% 95.7M 2s
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  8000K .......... .......... .......... .......... .......... 13% 81.7M 2s
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 10000K .......... .......... .......... .......... .......... 17%  159M 1s
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 15650K .......... .......... .......... .......... .......... 26%  183M 1s
 15700K .......... .......... .......... .......... .......... 26%  217M 1s
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 16850K .......... .......... .......... .......... .......... 28% 10.9M 1s
 16900K .......... .......... .......... .......... .......... 28% 5.82M 1s
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 17000K .......... .......... .......... .......... .......... 28% 14.8M 1s
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 17100K .......... .......... .......... .......... .......... 29% 7.22M 1s
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 20800K .......... .......... .......... .......... .......... 35% 5.88M 1s
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 25300K .......... .......... .......... .......... .......... 43% 79.2M 1s
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INFO: Download finished! File saved here: scarf_datasets/kang_14K_ifnb-pbmc_rnaseq/data.zarr.tar.gz
...... 97%  645K 0s
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 58850K .......... .......... .......... ........             100%  227M=1.3s

2021-08-22 17:58:12 (42.8 MB/s) - ‘scarf_datasets/kang_14K_ifnb-pbmc_rnaseq/data.zarr.tar.gz’ saved [60301820/60301820]

time: 16.7 s (started: 2021-08-22 17:57:57 +00:00)
[3]:
# Control/untreated PBMC data
ds_ctrl = scarf.DataStore('scarf_datasets/kang_15K_pbmc_rnaseq/data.zarr', nthreads=4)
ds_ctrl.plot_layout(layout_key='RNA_UMAP', color_by='cluster_labels')
../_images/vignettes_data_projection_4_0.png
time: 1.95 s (started: 2021-08-22 17:58:14 +00:00)
[4]:
# Interferon beta stimulated PBMC data
ds_stim = scarf.DataStore('scarf_datasets/kang_14K_ifnb-pbmc_rnaseq/data.zarr', nthreads=4)
ds_stim.plot_layout(layout_key='RNA_UMAP', color_by='cluster_labels')
../_images/vignettes_data_projection_5_0.png
time: 1.15 s (started: 2021-08-22 17:58:16 +00:00)

2) K-Nearest Neighbours (KNN) mapping

The run_mapping method of DataStore class performs KNN mapping/projection of target cells over reference cells. Reference cells are the ones that are present in the object where run_mapping is being called. The Assay object of target cells is provided as an argument to run_mapping. This step will load the latest graph of the reference cells and query the Approximate Nearest Neighbour (ANN) index of the reference cells for nearest neighbours of each target cell. Since the ANN index doesn’t contain any target cells, nearest neighbours of target cells will exclusively be reference cells. Under the hood, run_mapping method makes sure that the feature order in the target cells is same as that in the reference cells. By default, run_mapping will impute zero values for missing features in the target order to preserve the feature order. Here we have set run_coral parameter to True which activates CORAL normalization of target data. CORAL aligns the the feature distribution between reference and target cells thus removing systemic difference between reference and target cells. Read more about CORAL here. Here we use control PMBCs as reference because we invoke run_mapping on control PBMCs’ DataStore object and provide stimulated PBMC’s RNA assay as target.

Reference cells: The cells from the dataset that forms the basis of mapping. A KNN graph must already be calculated for this dataset.

<b>Target cells</b>: The cells to be projected onto reference cells. This dataset is not required to have a graph calculated.

[5]:
# CORAL algorithm can be very slow with large number of features (> 5000).
# Hence here it is recommended for only scRNA-Seq datasets.

ds_ctrl.run_mapping(target_assay=ds_stim.RNA, target_name='stim',
                    target_feat_key='hvgs_ctrl', save_k=5, run_coral=True)
INFO: 0 features missing in target data
INFO: Normalizing with feature subset
[########################################] | 100% Completed |  2.6s
Writing aligned normed target data to RNA/normed__I__hvgs_ctrl/data: 100%|██████████| 8/8 [00:04<00:00,  1.74it/s]
INFO: 2000 features being used for mapping
INFO: No value provided for parameter `log_transform`. Will use previously used value: True
INFO: No value provided for parameter `renormalize_subset`. Will use previously used value: True
INFO: No value provided for parameter `dims`. Will use previously used value: 25
INFO: No value provided for parameter `pca_cell_key`. Will use previously used value: I
INFO: Using PCA for dimension reduction
INFO: No value provided for parameter `ann_metric`. Will use previously used value: l2
INFO: No value provided for parameter `ann_efc`. Will use previously used value: 50
INFO: No value provided for parameter `ann_ef`. Will use previously used value: 50
INFO: No value provided for parameter `ann_m`. Will use previously used value: 48
INFO: No value provided for parameter `rand_state`. Will use previously used value: 4466
INFO: No value provided for parameter `k`. Will use previously used value: 11
INFO: No value provided for parameter `n_centroids`. Will use default value: 100
INFO: No value provided for parameter `local_connectivity`. Will use previously used value: 1.0
INFO: No value provided for parameter `bandwidth`. Will use previously used value: 1.5
INFO: Using existing normalized data with cell key I and feat key I__hvgs
INFO: Using existing loadings for pca with 25 dims
INFO: Using existing ANN index
INFO: using existing kmeans cluster centers

INFO: KNN graph already exists will not recompute.
INFO: INFO: (CORAL) Calculating source feature means
[########################################] | 100% Completed |  0.4s
INFO: INFO: (CORAL) Calculating source feature stdev
[########################################] | 100% Completed |  0.5s
INFO: INFO: (CORAL) Calculating target feature means
[########################################] | 100% Completed |  0.4s
INFO: INFO: (CORAL) Calculating target feature stdev
[########################################] | 100% Completed |  0.5s
INFO: CORAL: Computing source covariance
[########################################] | 100% Completed |  3.3s
INFO: CORAL: Computing target covariance
[########################################] | 100% Completed |  2.9s
INFO: Calculating fractional power of covariance matrices. This might take a while...
INFO: Fractional power calculation complete
Writing out coral corrected data: 100%|██████████| 11/11 [00:04<00:00,  2.74it/s]
Mapping: 11it [00:01,  7.21it/s]
time: 1min 59s (started: 2021-08-22 17:58:17 +00:00)


3) Mapping scores

We can use mapping scores to perform cross dataset cluster similarity inspection. mapping scores are scores assigned to each reference cell based on how frequently it was identified as one of the nearest neighbour of the target cells. get_mapping_score method allows generating these scores. We use an optional parameter of get_mapping_score, target_groups. target_groups takes grouping information for target cells such that mapping scores are calculated for one group at a time. Here we provide the cluster information of stimulated cells as group information and mapping scores will be obtained for each target cluster independently. The UMAPs below show how much mapping score each control cell received upon mapping from cells from one of the IFN-B stimulated cell clusters

[6]:
# Here we will generate plots for IFB-B stimulated cells from NK  and CD14 monocyte clusters.

for g, ms in ds_ctrl.get_mapping_score(target_name='stim',
                                       target_groups=ds_stim.cells.fetch('cluster_labels'),
                                       log_transform=True):
    if g in ['NK', 'CD 14 Mono']:
        print (f"Target cluster {g}")
        ds_ctrl.plot_layout(layout_key='RNA_UMAP', color_by='cluster_labels',
                            size_vals=ms*10, height=4, width=4, legend_onside=False)
Target cluster CD 14 Mono
../_images/vignettes_data_projection_11_1.png
Target cluster NK
../_images/vignettes_data_projection_11_3.png
time: 869 ms (started: 2021-08-22 18:00:17 +00:00)

4) Label transfer

Using the nearest neighbours of the target cells in the reference data, we can transfer labels from reference cells to target cells based on majority voting. This means that if a target cell has ‘most’ of its total edge weight shared with cells from one cell type, then that cell type label is tranferred to the target cell. The default threshold for ‘most’ is 0.5, i.e. half of all edge weight. get_target_classes method returns the transferred labels for each cell from a given mapped target dataset.

reference_class_group parameter decides which labels to transfer. This can be any column from the cell attribute table that has categorical values, generally users would use RNA_leiden_cluster or RNA_cluster but they can also use other labels. Here, for example, we use the custom labels stored under cluster_labels column.

[7]:
transferred_labels = ds_ctrl.get_target_classes(target_name='stim',
                                                reference_class_group='cluster_labels')
transferred_labels
[7]:
0                  NK
1                 pDC
2         CD4 naive T
3                   B
4         CD4 naive T
             ...
10106      CD 14 Mono
10107    CD4 Memory T
10108               B
10109      CD 14 Mono
10110     CD4 naive T
Length: 10111, dtype: object
time: 202 ms (started: 2021-08-22 18:00:17 +00:00)

We can now save these transferred labels into the stimulated cell dataset and visualize them of its UMAP.

[8]:
ds_stim.cells.insert('transferred_labels',
                     transferred_labels.values,
                     overwrite=True)
time: 42.1 ms (started: 2021-08-22 18:00:18 +00:00)
[9]:
ds_stim.plot_layout(layout_key='RNA_UMAP', color_by='transferred_labels')
../_images/vignettes_data_projection_16_0.png
time: 1.14 s (started: 2021-08-22 18:00:18 +00:00)

It can be quite interesting to check how the predicted/transferred labels compare to the actual labels of the target cells.

[10]:
import pandas as pd

df = pd.crosstab(ds_stim.cells.fetch('cluster_labels'),
                 ds_stim.cells.fetch('transferred_labels'))
df
[10]:
col_0 B B activated CD 14 Mono CD16 Mono CD4 Memory T CD4 naive T CD8 T DC Eryth Mk NA NK T activated pDC
row_0
B 649 26 19 0 15 20 0 1 0 4 3 1 6 2
B activated 24 171 3 0 1 2 1 0 0 0 2 1 0 0
CD 14 Mono 0 0 2381 132 3 1 0 7 0 6 2 0 0 0
CD16 Mono 1 0 230 717 0 0 0 1 0 7 0 0 0 0
CD4 Memory T 5 0 45 1 923 217 72 0 1 5 18 0 10 0
CD4 naive T 14 4 36 0 356 1896 16 2 1 16 19 2 28 0
CD8 T 10 0 23 0 59 14 461 0 0 2 5 76 5 0
DC 0 0 73 4 0 0 0 137 0 0 0 0 0 0
Eryth 0 0 11 0 1 23 1 0 46 0 1 0 0 0
Mk 1 0 46 1 17 25 1 2 2 149 0 0 3 0
NK 1 0 9 0 3 0 37 0 0 2 1 376 1 0
T activated 2 1 4 0 34 24 1 1 0 1 0 0 214 0
pDC 0 0 1 0 0 1 0 3 0 0 0 1 0 68
time: 52.3 ms (started: 2021-08-22 18:00:19 +00:00)

This cross-tabulation can be presented as percentage accuracy, where the values indicate the percentage of the transferred values that were correct.

[11]:
(100 * df / df.sum(axis=0)).round(1)
[11]:
col_0 B B activated CD 14 Mono CD16 Mono CD4 Memory T CD4 naive T CD8 T DC Eryth Mk NA NK T activated pDC
row_0
B 91.8 12.9 0.7 0.0 1.1 0.9 0.0 0.6 0.0 2.1 5.9 0.2 2.2 2.9
B activated 3.4 84.7 0.1 0.0 0.1 0.1 0.2 0.0 0.0 0.0 3.9 0.2 0.0 0.0
CD 14 Mono 0.0 0.0 82.6 15.4 0.2 0.0 0.0 4.5 0.0 3.1 3.9 0.0 0.0 0.0
CD16 Mono 0.1 0.0 8.0 83.9 0.0 0.0 0.0 0.6 0.0 3.6 0.0 0.0 0.0 0.0
CD4 Memory T 0.7 0.0 1.6 0.1 65.4 9.8 12.2 0.0 2.0 2.6 35.3 0.0 3.7 0.0
CD4 naive T 2.0 2.0 1.2 0.0 25.2 85.3 2.7 1.3 2.0 8.3 37.3 0.4 10.5 0.0
CD8 T 1.4 0.0 0.8 0.0 4.2 0.6 78.1 0.0 0.0 1.0 9.8 16.6 1.9 0.0
DC 0.0 0.0 2.5 0.5 0.0 0.0 0.0 89.0 0.0 0.0 0.0 0.0 0.0 0.0
Eryth 0.0 0.0 0.4 0.0 0.1 1.0 0.2 0.0 92.0 0.0 2.0 0.0 0.0 0.0
Mk 0.1 0.0 1.6 0.1 1.2 1.1 0.2 1.3 4.0 77.6 0.0 0.0 1.1 0.0
NK 0.1 0.0 0.3 0.0 0.2 0.0 6.3 0.0 0.0 1.0 2.0 82.3 0.4 0.0
T activated 0.3 0.5 0.1 0.0 2.4 1.1 0.2 0.6 0.0 0.5 0.0 0.0 80.1 0.0
pDC 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.9 0.0 0.0 0.0 0.2 0.0 97.1
time: 44.9 ms (started: 2021-08-22 18:00:19 +00:00)

5) Unified UMAPs

Scarf introduces Unified UMAPs, a strategy to embed target cells onto the reference manifold. To do so, we take the results of KNN projection and spike the graph of reference cells with target cells. We can control the weight of target-reference edges also and also the number of edges per target to retain. We rerun UMAP on this ‘unified graph’ to obtain a unified embdding. Following code shows how to call run_unified_umap method.

[12]:
ds_ctrl.run_unified_umap(target_names=['stim'], ini_embed_with='RNA_UMAP', target_weight=1,
                         use_k=5, fit_n_epochs=100, tx_n_epochs=10)
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time: 24 s (started: 2021-08-22 18:00:19 +00:00)

Since the results of unified embedding contain ‘foreign’ cells, plot_layout function cannot be used to visualize all the cells. A specialized method, plot_unified_layout takes care of this issue. The following example shows co-embedded control (reference) and stimulated (target) PBMCs.

[13]:
ds_ctrl.plot_unified_layout(layout_key='unified_UMAP', show_target_only=False, ref_name='ctrl')
../_images/vignettes_data_projection_24_0.png
time: 973 ms (started: 2021-08-22 18:00:43 +00:00)

We can visualize only the target cells, i.e IFN-B stimulated cells, in the unified embedding. The target cells can be colored based on their original cluster identity. Target cells of similar types are close together on the unified embedding and overlap with the cell types of the reference data

[14]:
ds_ctrl.plot_unified_layout(layout_key='unified_UMAP', show_target_only=True, legend_ondata=True,
                            target_groups=ds_stim.cells.fetch('cluster_labels'))
../_images/vignettes_data_projection_26_0.png
time: 1.29 s (started: 2021-08-22 18:00:44 +00:00)

That is all for this vignette.